|Tel :||01904 431402||Bioquote Limited|
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York, YO10 3DW
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|Int Tel :||00 44 1904 431402|
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Manufacturer: Biomedical Technologies Inc
Shipping: Cold Gel Packs
Catalog No: BT-567
Lot No: 5671108
Packaging: BTI rabbit, anti-Non Muscle Myosin IIA antibody is packaged in 0.5ml aliquots with 0.02% Sodium Azide added as preservative. The Rabbit IgG concentration is 8 mg/ml.
Storage & Stability: Stable for one year at -80°C. Refrigerated at 4°C it is stable for six months.
Preparation: Rabbits were immunized with myosin II purified (95% SDS PAGE) from macrophage-like J774 cells as described 1.
Specificity: BTI anti-non muscle Myosin IIA binds the heavy chain, non-muscle myosin IIA in vertebrates (1). It contains no detectable cross-reactivity with any other protein including the following:
Myosin II skeletal.
Myosin II-smooth muscle.
Myosin II-cardiac muscle.
Myosin IIB-non-muscle 2.
Western Blots: Whole cell extracts (in ice) should be boiled in SDS sample buffer. Apply 10-25ug Protein to each lane of SDS acrylamide gels, electrophorese and transfer to nitrocellulose, incubate overnight at 10°C with this antibody (1:5000) in TBS with 1% BSA and then envisioned with GAR IgG HRP. Develop the blot using ECL. Expose for 15-60 seconds.
A complete protocol is available.
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