|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
|Fax :||01904 431409|
|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Manufacturer: BioCellChallenge SAS
Red Calcium Phosphate Transfection Kit Advantages :
The economical way to transfect very efficiently HEK293.
Better stability & reproducibility.
High cell viability.
Easy to handle.
Stable & transient transfections.
Recombinant protein production.
Red Calcium Phosphate Transfection Kit :
A standard for high level of protein expression and virus production in HEK cells.
The Red Calcium Phosphate Transfection Kit is based on Graham and Van Der Eb method published in 1973. This method allows to introduce DNA inside cells and works best with highly transformed adherent cell lines such as HEK293. However, other cells and cell lines can be transfected with this method. If its transfection efficiency is not as high as the one obtain by using last generations of cationic polymers and cationic lipids it presents several advantages including very low cytotoxicity with sensitive cells.
Tedious optimizations are required and reproducibility problems can occur when using this method for DNA transfection. The Red Calcium Phosphate Transfection Kit we developped was improved in order to increase its stability and reproducibility. This kit is dedicated for producing proteins and viruses in HEK cells but can also be used to transfect sensitive cells.
45 mL of HBS 1X (ready to use).
3.2 mL of 2M Calcium chloride.
Catalogue Number : RC75
Allows to perform 75 transfections in 10 cm dishes.
All prices shown are exlusive of VAT.