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Tubulin polymerization HTS assay using >97% pure tubulin, OD based - Porcine (BK004P)

Manufacturer: Cytoskeleton Inc
Shipping: Standard

Product Uses Include :

  • Screening compounds for effects on tubulin polymerization activity.
  • Screening proteins for effects on tubulin polymerization activity.
  • Teaching aid for undergraduate/graduate class in pharmacology.
  • Economical approach to HTS with tubulin.
     

Introduction :
This assay is based on an adaptation of the original method of Shelanski et al. and Lee et al. (1,2), which demonstrated that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The resulting polymerization curve is representative of the three phases of microtubule polymerization, namely nucleation, growth and steady state equilibrium. See the About Tubulin page for more information. The assay is optimized for a 96-well format for low CVs and large sample number handling.

This kit contains "HTS tubulin", which is >97% pure (Cat. # HTS03). The same assay is also available with our highest purity tubulin (>99% pure, Cat. # T240) and is sold under Cat. # BK006P. BK004P provides an economical alternative to the higher purity BK006P assay and can be a good choice for large primary screens. Cytoskeleton, Inc. also provides a fluorescence based tubulin polymerization assay in miniaturized format (Cat. # BK011P), which is also ideal for high throughput screening.

If you are interested in using either of these tubulin polymerization assays in a high throughput setting, please contact our technical service department for advice and bulk quotes.

Kit contents :
This kit contains enough materials for 24 assays (BK004P). The following reagents are included:

  1. HTS tubulin, lyophilized (Cat. # HTS03)
  2. GTP solution (Cat. # BST06)
  3. General tubulin buffer (PEM, Cat. # BST01)
  4. Tubulin glycerol buffer (Cat. # BST05)
  5. Paclitaxel positive control (Cat. # TXD01)
  6. DMSO for paclitaxel.
  7. Half area 96-well plate for polymerization reactions
  8. Manual with detailed protocols and extensive troubleshooting guide.
     

Equipment needed :

  1. 96-well plate spectrophotometer with filters to read optical density at 340 nm.
  2. Multi-channel pipette for rapid pipetting of tubulin

References :

  1. Shelanski, M. L., Gaskin, F. and Cantor, C. R. (1973). Microtubule assembly in the absence of added nucleotides. Proc. Natl. Acad. Sci. U.S.A. 70, 765-768.
  2. Lee, J. C. and Timasheff, S. N. (1977). In vitro reconstitution of calf brain microtubules: effects of solution variable. Biochemistry, 16, 1754-1762.

 

SizeCatalogue noPrice 
Kit size BK004P £471.00/€659.40
Qty:

All prices shown are exlusive of VAT.