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Manufacturer: bioWorld Inc
Recombinant Rat Receptor Associated Protein (RAP)
Source: E. Coli
Background: Receptor-associated protein (RAP) functions as a specialized endoplasmic reticulum chaperone assisting in the folding and trafficking of members of the low-density lipoprotein (LDL) receptor family. RAP efficiently binds to these receptors and antagonizes binding of other ligands.
Description : Recombinant Rat Receptor Associated Protein is a fused-Rat his:RAP:c-myc protein expressed in E.Coli with a MW of 40kD (38862 Daltons) as determined by SDS gel electrophoresis. Recombinant RAP is purified by proprietary chromatographic techniques.
Physical Appearance: White lyophilized (freeze-dried) powder.
Formulation: Recombinant RAP, at a concentration of 1 mg/ml, was lyophilized from a sterile solution containing TBS pH-7.5, 0.1% BSA and 0.09% NaN3.
Solubility: It is recommended to reconstitute the lyophilized recombinant RAP in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized RAP although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution RAP should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.
Dimers and aggregates: Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.
Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Human Keratin.
Usage: BioWORLD\'s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Storage : -20°C
Size : 5 µg
Catalogue No. : 22060674-1
All prices shown are exlusive of VAT.