|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
|Fax :||01904 431409|
|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Shipping: Dry Ice
Product Subcategory : Tissue Lysate
Protein Family :
Pathway & Disease :
Description : Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Format : Lysate is supplied at 2 mg/ml in 1X SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Applications : WB
Data Legend :
Product Citations :
Recommended Products :
Alternate Names : total protein normal small intestine - human
Accession Number :
Link To Accession Number Web :
Application Notes : WB: Boil the mixture for 10 min before loading. Load 5-20 ug lysate per lane.
Storage and Stability : Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -80°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.
|0.1 mg size||450241||£210.00/€294.00|
All prices shown are exlusive of VAT.