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Manufacturer: Cytoskeleton Inc
Product Uses Include :
This assay is a one step procedure for performing plant tubulin polymerization assays. Polymerization is followed by fluorescence enhancement due to the incorporation of a fluorescent reporter into microtubules as polymerization occurs. It is an adaptation of an assay originally described by Bonne, D. et al. (1) for bovine brain tubulin. By calculating Vmax of polymerization in AFU/min you can determine the specific activity of a compound or protein on plant tubulin polymerization.
Example results :
Compounds or proteins that interact with tubulin will often alter one or more of the characteristic phases of polymerization. For example, Figure 1 shows the effect of adding the anti-mitotic drug paclitaxel to the standard polymerization reaction. A 3 µM concentration of paclitaxel eliminates the nucleation phase and enhances the Vmax of the growth phase. Thus, one application of this assay is the identification of novel anti-mitotic compounds that enhance polymerization and stabilize microtubules. Figure 1 also shows the effect of adding the tubulin binding drug, trifluran. At 3 µM final concentration, trifluran causes a drastic decrease in Vmax and reduction in final polymer mass.
Kit contents :
The kit contains sufficient material for 24 assays in 10 µl format. The following components are included:
Equipment needed :
Bonne, D., Heusele, C., Simon, C., and Pantaloni, D. (1985). 4’, 6-Diamidino-2-phenylindole, a fluorescent probe for tubulin and mictrotubules. J. Biol. Chem. 260, 2819-2825.
|30-36 Assays Kit size||BK010S||£1,295.00/€1,813.00|
All prices shown are exlusive of VAT.