|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
|Fax :||01904 431409|
|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Manufacturer: Cytoskeleton Inc
NOTE : THIS PRODUCT IS NO LONGER AVAILABLE AND HAS BEEN REPLACED BY PRODUCT CODES CT04-A AND CT04-B
TYPE IN PRODUCT CODE CT04-A OR CT04-B IN THE SEARCH BOX
Product Uses Include :
The G-switch™ line of small G-protein tools has been developed with an emphasis on creating highly potent reagents that target endogenous Rho family proteins and pathways. In contrast to methods that rely on over-expression or knockdown of target proteins (e.g., DNA transfection of dominant negative or constitutively active Rho mutants, RNAi knockdown) , the G-switchTM reagents act rapidly on the endogenous target protein (in minutes to hours, depending on product), thereby optimizing the chance of generating a more physiologically relevant response. The G-switch™ product line includes reagents that directly and indirectly modulate Rho family signal transduction, thereby offering a wide range of mechanistic tools to study these critical cellular functions. See Cytoskeleton’s web site for the latest G-switch™ information.
Rho Pathway Inhibitor CN06 acts by directly and potently inhibiting the Rho-associated coiled-coil forming protein serine/threonine kinase (ROCK) family of proteins, with a Ki of 220 nM for ROCK I and 300 nM for ROCK II (1,2). The affinities for other Rho effector kinases; such as citron and PKN are at least 10X lower (1). Effector kinases in the Rho family pathway such as the Rac/Cdc42 effector, p21-activated kinase (PAK), are not affected (3). The uptake of cell permeable CN06 is time and temperature dependent. Uptake has been shown to reach a plateau at 30 minutes at 37°C and is negligible at 4°C (4). ROCK proteins are ubiquitously expressed, making this inhibitor applicable to the study of almost all cell lines and tissues (4 and Table 1).
|5x10 units size||CN06-A||£115.00/€161.00|
|20 x 10 units size||CN06-B||£295.00/€413.00|
All prices shown are exlusive of VAT.