|Tel :||01904 431402||Bioquote Limited|
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York, YO10 3DW
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|Int Tel :||00 44 1904 431402|
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Manufacturer: Cytoskeleton Inc
The principle of the assay is shown schematically below (Figure 1). The assay is based upon the fact that a Rho family effector protein is known to bind preferentially to the active (GTP-bound) form of its target GTPase (1). In the case of RhoA activation, the rhotekin-RBD effector domain is used to make the affinity beads (2). In the case of Cdc42 and Rac1 activation, the PAK-PBD effector domain is used for affinity beads (3). Example Western blot results and detailed protocols can be viewed by clicking the documents tab above and downloading the pdf datasheet.
The kit contains sufficient materials for 10 assays each for RhoA, Rac1, and Cdc42 (depending on activation levels in cells), including reagents for positive and negative controls. The following components are included:
|Combo Kit size||BK030||£689.00/€964.60|
All prices shown are exlusive of VAT.