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Manufacturer: Cytoskeleton Inc
The Anti-acetyl Lysine Antibody recognizes proteins post-translationally modified by acetylation on the epsilon amine groups of lysine residues. A proprietary mixture of acetylated proteins was used to produce a highly robust antibody that has been shown to recognizes a wide range of acetylated proteins in IP, WB, ChIP and IF applications. This Anti-acetyl Lysine Antibody has many advantages when compared to other commercially available antibodies as shown below.
Host/Isotype : Mouse / IgG2b
Clone : 3C6.08.20
Species Reactivity : All Species
Applications : WB, IPP, IF, ChIP
Protein Acylation Background :
Acetylation of proteins can occur as a co-translational or post-translational modification (PTM) (1). Co-translational acetylation occurs at the N-terminal of approximately 85% of mammalian proteins, it is irreversible and is thought to be important in protein stability, localization and synthesis (1). Post-translational acetylation occurs on the epsilon amino group of lysine residues as a reversible and highly dynamic PTM that is known to be a key regulator in multiple cellular events, including chromatin structure, transcription, metabolism, signal transduction and cytoskeletal regulation (2-3). To date over 4,000 proteins have been identified as targets for PTM acetylation which is comparable to phosphorylation in cellular prevelance (3). Antibody AAC01 detects acetyl lysine PTMs.
1 Bogdan P. and Sherman F. 2002. The diversity of acetylated proteins. Genome Biol. 3 (5): reviews 0006.
2 Lundby A. et al. 2012. Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and cellular patterns. Cell Reports 2:419-431.
3 Sadoul K. et al. 2010. The tale of protein lysine acetylation in the cytoplasm. J. Biomed. Biotech. 2011:1-15.
4 Golemis EA et. Al, Protein-Protein Interactions, CSHLP, 2005, p67
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