|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
|Fax :||01904 431409|
|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Manufacturer: Cytoskeleton Inc
Material Cardiac myosin protein has been purified from bovine heart tissue (1,2,3). The full length myosin protein was purified with its essential light chains (ELC) and regulatory light chains (RLC), see Figure 1 and 2. Myosin was then digested with -chymotrypsin in the presence of MgCl2 to liberate the soluble heavy fragment (HMM) domain, which was isolated by centrifugation followed by anionic exchange chromatography to remove S1 (2,3). The purified myosin HMM fragment has been determined to be biologically active in an F-actin activated ATPase assay (see biological activity assay). Bovine cardiac myosin S1 fragment protein is supplied as a white lyophilized powder.
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