|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
|Fax :||01904 431409|
|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Manufacturer: Viagen Biotech Inc
Description : Proteinase K is an endoproteolytic enzyme that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a nonspecific serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.
Applications : Proteinase K effectively digests native proteins. It can be used to inactivate DNases and RNases when isolating DNA and RNA from tissues or cells.
• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At high temperatures—optimum digestion temperature is 65°C
Unit Definition : One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.
Quality Control : Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases
All prices shown are exlusive of VAT.