|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
|Fax :||01904 431409|
|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Manufacturer: ArborAssays Inc
Shipping: Cold Gel Packs
The DetectX® Endothelin-1 (ET-1) Immunoassay kit is designed to quantitatively measure ET-1 present in a variety samples and tissue culture media. An ET-1 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture ET-1 present in the sample. After a 60 minute incubation, the plate is washed and a peroxidase conjugated ET-1 antibody is added. The plate is again incubated for 60 minutes and washed. Substrate is then added to the plate, which reacts with the bound ET-1 conjugated antibody. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the ET-1 in the sample is then calculated.
|1x96 Well Plate Kit size||K045-H1||£475.00/€665.00|
All prices shown are exlusive of VAT.