|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
|Fax :||01904 431409|
|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Manufacturer: NSJ Bioreagents
Shipping: Cold Gel Packs
Family : Antibody
Formulation : 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format : Purified
Clone : VM452
Host Animal : Mouse
Clonality : Monoclonal (mouse origin)
Isotype : Mouse IgG1
Species Reactivity : Human, Cow, Dog, Cat, Pig, Goat and Chicken. Does not react with mouse and rat. Other species not known.
Application : WB, FACS, IHC, IF
Application Details : FACS: 0.5-1ug/million cells,IF: 1-2ug/ml,WB: 0.5-1ug/ml,IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)
Application Note : <p>The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.</p><p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.</br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen : Recombinant protein was used as the immunogen for the Vimentin antibody.
Storage : Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
|100 ug size||V2296-100UG||£329.00/€460.60|
|20 ug size||V2296-20UG||£129.00/€180.60|
All prices shown are exlusive of VAT.