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Manufacturer: Cytoskeleton Inc
The human SOS1 exchange domain (564-1049) protein (SOS1-ExD) has been produced in a bacterial expression system. The recombinant protein contains a 6 histidine tag (His-tag) at its amino terminus. The approximate molecular weight of the SOS1-ExD protein is 61 kDa. The protein is supplied as a lyophilized white powder.
The protein should be reconstituted to 50 µM (3.03 mg/ml) by the addition of 33 μl of distilled water. The protein will be in the following buffer: 17 mM Tris pH 7.5, 17 mM NaCl, 0.2 mM MgCl2, 2.3% sucrose, and 0.3% dextran. In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be aliquoted into "experiment sized" aliquots and snap frozen in liquid nitrogen. The protein can be stored at -70°C for 6 months. Avoid repeated freeze-thaw cycles. The lyophilized protein is stable at 4°C for 1 year when stored desiccated (<10% humidity).
Protein purity is determined by scanning densitometry of Coomassie blue stained protein on a 4-20% polyacrylamide gradient gel. The SOS1-ExD protein was determined to be >90% pure. (see Figure 1).
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