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Human SOS1 Protein (Exchange Domain 564-1049)

Manufacturer: Cytoskeleton Inc
Shipping: Standard

Product Uses

  • Study inhibitors of SOS1 GTP exchange activity
  • Identification of SOS1 binding proteins
  • Study of SOS1 GEF activity with different GTPases

Materials

The human SOS1 exchange domain (564-1049) protein (SOS1-ExD) has been produced in a bacterial expression system. The recombinant protein contains a 6 histidine tag (His-tag) at its amino terminus. The approximate molecular weight of the SOS1-ExD protein is 61 kDa. The protein is supplied as a lyophilized white powder.

Storage

The protein should be reconstituted to 50 µM (3.03 mg/ml) by the addition of 33 μl of distilled water. The protein will be in the following buffer: 17 mM Tris pH 7.5, 17 mM NaCl, 0.2 mM MgCl2, 2.3% sucrose, and 0.3% dextran. In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be aliquoted into "experiment sized" aliquots and snap frozen in liquid nitrogen. The protein can be stored at -70°C for 6 months. Avoid repeated freeze-thaw cycles. The lyophilized protein is stable at 4°C for 1 year when stored desiccated (<10% humidity).

Purity

Protein purity is determined by scanning densitometry of Coomassie blue stained protein on a 4-20% polyacrylamide gradient gel. The SOS1-ExD protein was determined to be >90% pure. (see Figure 1).

SizeCatalogue noPrice 
1x100ug size CS-SOS1-A £299.00/€418.60
Qty:
1x1mg size CS-SOS1-B £1,319.00/€1,846.60
Qty:

All prices shown are exlusive of VAT.