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Arginine Separopore 4B

Manufacturer: bioWorld Inc
Shipping: Cold Gel Packs

Application: Arginine-Separopore® 4B is used for purification of molecules with biospecific or charge dependent affinity for Arginine. It is also used for the purification of lysine-binding proteins from serum and to purify plasminogen, plasminogen activator, ribosomal RNA, and double-stranded DNA.

Specifications:

Ligand: Arginine

Particle size range: 52 – 165 µm

Molecular weight range: 6x104 - 2x107

Matrix: Separopore® 4B (agarose beads, 4%)

Matrix active group: Cyanogen bromide Attachment: α-Amino

Extent of coupling: ~4 μmol / ml

pH stability: 4 – 9

Flow specifications: 70 – 140 cm / h

Supplied as a suspension in 2M NaCl, 0.02% thimerosal

Storage temp.: 2 – 8 °C

References:

Expression analysis and purification of human recombinant tissue type plasminogen activator (rt-PA) from transgenic tobacco plants. Prep Biochem Biotechnol. (2011) 41: 175-86.

Simplified recombinant plasmin: production and functional comparison of a novel thrombolytic molecule with plasma-derived plasmin. Thromb Haemost. (2008) 100: 413-9.

Glycosylation variant analysis of recombinant human tissue plasminogen activator produced in urea-cycle-enzyme-expressing Chinese hamster ovary (CHO) cell line. J Biosci Bioeng. (2006) 102: 447-51.

Baboon lipoprotein(a) binds very weakly to lysine-agarose and fibrin despite the presence of a strong lysine-binding site in apolipoprotein(a) kringle IV type 10. Biochemistry. (2005) 44: 555-64.

Anti-angiogenic role of angiostatin during corneal wound healing. Exp Eye Res. (2004) 78: 579-89.

Angiostatin and matrix metalloprotease expression following ischemic acute renal failure. Am J Physiol Renal Physiol. (2004) 286: F893-902. A new method of purifying fibrinogen with both biological and immunological activity from human plasma. Prep Biochem Biotechnol. (2003) 33: 239-52.

Generation and role of angiostatin in human platelets. Blood. (2003) 102: 3217-23. Identification of a novel apolipoprotein(a)-related protein from the European hedgehog (Erinaceus europaeus). DNA Seq. (2003) 14: 15-23.

Malignant ascites fluid (MAF), including ovarian-cancer-associated MAF, contains angiostatin and other factor(s) which inhibit angiogenesis. Gynecol Oncol. (2002) 86: 279-87.

Angiostatin II is the predominant glycoform in pleural effusates of rabbit VX-2 lung tumors. J Lab Clin Med. (2002) 139: 316-23.

Antifibrinolytic effect of single apo(a) kringle domains: relationship to fibrinogen binding. Protein Eng. (2001) 14: 427-38.

Purification and partial characterisation of alpha(2)-antiplasmin and plasmin(ogen) from ostrich plasma. Comp Biochem Physiol B Biochem Mol Biol. (2001) 129: 809-20.

Identification of calnexin as a binding protein for Amadori-modified glycated albumin. Biochem Biophys Res Commun. (2001) 284: 602-6.

Properties of human free apolipoprotein(a) and lipoprotein(a) after either freezing or lyophilization in the presence and absence of cryopreservatives. Anal Biochem. (2001) 288: 201-8.

SizeCatalogue noPrice 
10ml size 20181125-1 £195.00/€273.00
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25ml size 20181125-2 £395.00/€553.00
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100ml size 20181125-3 £995.00/€1,393.00
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All prices shown are exlusive of VAT.