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Anti-Cdc42: Mouse MAb

Manufacturer: Cytoskeleton Inc
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Anti-Cdc42: Mouse MAb

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Introduction :
Cdc42 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Cdc42 is expressed in a large number of different cell types.

Product Uses Include :

This antibody is recommended for detection of Cdc42 in human, mouse, rat or other extracts.  The following protocols have been tested with this antibody: 

 

Western Blot : YES

Immunocytochemistry : YES

ELISA : NO

Immunoprecipitation : NO 

Material :
The anti-Cdc42 antibody (Cat. # ACD03) is a mouse monoclonal antibody that only recognizes Cdc42, not Rac1, Rac2, Rac3, RhoA, RhoB, RhoC or H-Ras. Other commercially available Cdc42 antibodies recognize one or more of these close isoforms  Platelet extract (Cat. # EXT01) is included as a positive control. ACD03 is supplied as a lyophilized white powder.

Figure 1 (Above) : Western blot analysis of purified protein and cell extracts using ACD03 as primary antibody. Recombinant small G-proteins and tissue extracts were separated by SDS-PAGE and transferred to PVDF membrane.  ACD03 was diluted to 250 ng/ml (1:1000) in PBST plus 0.1% non-fat milk powder and Western analysis was as described in the Methods section of the datasheet. Lane 1; 25 ng Cdc42-6xHIS, Lane 2; 250 ng RhoA-6xHIS, Lane 3; 250 ng Rac1-6xHIS, Lane 4; 40 µg Swiss 3T3 cell extract, Lane 5; 40 µg bovine brain extract, Lane 6; 40 µg human platelet extract. Note: Cdc42-6xHIS runs at 25kDal whereas the native Cdc42 runs at 22kDal.

SizeCatalogue noPrice 
2x200ul size ACD03 £230.00/€322.00
Qty:
1x50ul size ACD03-S £65.00/€91.00
Qty:

All prices shown are exlusive of VAT.