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Human PGR Reporter Assay System, 1 x 96-well format assay

Manufacturer: Indigo Biosciences
Shipping: Dry Ice

Product Overview :
This nuclear receptor assay system uses specific human cells modified to provide constitutive, high-level expression of the full-length human progesterone receptor (NR3C3), a ligand-dependent transcription factor commonly referred to as PR. Indigo’s reporter cells include the luciferase reporter gene functionally linked to a PR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in PR activity. Luciferase gene expression occurs after ligand-bound PR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the target gene. Unlike in vitro binding assays, and some other cell-based assay strategies, the readout from Indigo’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. PR reporter cells are prepared using Indigo’s specific CryoMite™ process. This cryopreservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. Indigo Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to PR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. IB0500

Size : 1 Kit        

Shipping : Dry Ice

Stability : Store at -80 degrees; shelf life 180 days maximum after production.

Application(s) : human

Specificity : Progesterone Receptor (PGR; NR3C3)

Custom Code : 3822000002

UNSPSC code : 41116104

 

SizeCatalogue noPrice 
1x96 Well Plate size IB05001 £775.00/€1,085.00
Qty:

All prices shown are exlusive of VAT.