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Iso-Gold Rapid Mouse - Monoclonal Isotyping Kit

Manufacturer: BioAssayWorks LLC
Shipping: Cold Gel Packs

Iso-Gold Rapid Mouse - Monoclonal Isotyping Kit

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Iso-Gold™ Rapid Mouse -Monoclonal Isotyping Kit

NEW Options for Rapid Isotyping: BioAssay Works has developed a five-minute rapid mouse-monoclonal isotyping kit with ELISA sensitivity for Mab class and subclass determination. In less than 10 minutes the isotype is determined. In comparison, it may take a day and a half to set up and run the ELISA. Save a day of labor and deliver monoclonal identity results!

There are a number of ELISA-based isotyping kits on the market. These kits require the customer to supply their own plates, do the coating, incubation and kit development.  One latex-bead-based "rapid" isotyping kit is also marketed, but the sensitivity increase of a gold-sol-based assay versus a latex-based assay gives the advantage to the gold assay.

IsoGold Rapid-Mouse-Mononclonal Isotyping Kit is a rapid lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. The assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid.

IsoGold test kits are available in 5 (5) cassette pouch size or in ten (10) cassette pouch size. Store kit and components at room temperature – Shelf life 18 months.

Product Codes and Descriptions:

TISO-001 (Size-5 Test Pounches)

Iso-Gold Rapid Mouse-Monoclonal Isotyping kit
• 5 cassette pouches; 5 cassettes contain strips impregnated with anti-IgG1; anti-IgG2a;andanti-IgG2b isotypes; and 5 cassettes that contain strips that are impregnated with anti-IgG3, anti-IgM, and anti-IgA isotypes
• Sample Diluent  - 25 ml

ISOT-001 (Size-10 Test Pouches)

Iso-Gold Rapid Mouse-Monoclonal Isotyping kit
• 10 cassette pouches; ten (10) cassettes contain strips impregnated with anti-IgG1; anti-IgG2a;andanti-IgG2b isotypes; and ten (10) cassettes that contain strips that are impregnated with anti-IgG3, anti-IgM, and anti-IgA isotypes
• Sample Diluent  - 45 ml

Assay Utility: Determining the class and subclass of a mononclonal antibody is useful in determining the best immunoglobulin purification method.  For example, IgA and IgM are often best purified by size (gel exclusion) or on immuno affinity separation columns whereas IgG2a and IgG2b can be purified on protein A at a pH of 7 to 8.  IgG1 binds best to protein A at a pH of  8 to 9.  In addition, each class and isotype can be digested to Fab fragments using the appropriate amount of pepsin or other enzymes for each isotype or antibody class.

Assay background information:There are two (2) cassettes in each of the ten (ten) pouches: one cassette for detecting IgG1, IgG2a, and IgG2b; the other cassette detects IgG3; IgA, and IgM. When a properly diluted sample containing a specific isotype is added to the sample-well, specific-class and subclass soluble complexes are formed with the embedded gold conjugates. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Typically, when antibodies are tested at 10 nanograms per milliliter, results are read at five (5) to ten (10) minutes. (Results should not be read after ten (10) minutes.)

See BioAssay Works\' Application Notes or Technical Data for procedures for monoclonal antibody ascites fluid, for cell culture fluid, and for supernatant fluid.

SizeCatalogue noPrice 
Kit size TISO-001 £300.00/€420.00
Qty:
Kit size ISOT-001 £300.00/€420.00
Qty:

All prices shown are exlusive of VAT.