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Cofilin 2 Antibody

Manufacturer: Pro Sci
Shipping: Standard

Background

Cofilin 2 Antibody. This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)

Additional Names

CFL2; cofilin 2 (muscle) ; HGNC:1875 ; cofilin 2

DescriptionLeft: Western blot analysis of cofilin 2 in Human Muscle lysate (35μg protein in RIPA buffer) using cofilin 2 antibody (0.003μg/ml).

Source

Cofilin 2 antibody was raised against a synthetic peptide near the C-terminus of cofilin 2.

Purification

Cofilin 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

Cofilin 2 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 18kDa band observed in Human Duodenum, Heart and Muscle lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03µg/ml

Tested Application

E, WB

Buffer

Cofilin 2 antibody is supplied as 0.1mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Species Reactivity

H,M,R,D,P

Protein Accession Number

NP_068733.1; NP_619579.1

Data Sheet Version

08-01W

References

  1. Thirion C, Stucka R, Mendel B, Gruhler A, Jaksch M, Nowak KJ, Binz N, Laing NG, Lochmuller H. Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle. Eur J Biochem. 2001 Jun;268(12):3473-82

SizeCatalogue noPrice 
100ug size 45-036 £295.00/€413.00
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