|Tel :||01904 431402||Bioquote Limited|
The Raylor Centre
York, YO10 3DW
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|Int Tel :||00 44 1904 431402|
|Int Fax :||00 44 1904 431409|
Manufacturer: Biomedical Technologies Inc
The BTI Human Keratinocyte Transglutaminase MAb recognizes transglutaminase type 1 which is expressed during the differentiation of epidermis and stratified squamous epithelia. This MAb has been used for immunohistochemical localization on frozen tissues or formaldehyde (2%) fixed tissues and cells from human, hamster, monkey, rabbit and rat sources (paraffin-embedded sections do not stain properly). The BTI Transglutaminase MAb can be used in a modified elisa for the detection of type 1 transglutaminase expression in response to retinoids. This IgG2a antibody is purified via protein A sepharose. A complete protocol is included with the MAb, including methods for analyzing tissue sections and cultured cells.
1. Phillips, M.A; Stewart, B.E; Qin, Q; Chakravarty, R; Floyd, E; Jetten, A.M; and Rice, R.H. (1990) Primary Structure of Keratinocyte Transglutaminase. Proc. Natl. Acad. Sci. 87: 9333-9337.
2. Schmidt, R; Michel, S; Shroot, B; and U. Reichert. (1988) Transglutaminase in normal and transformed human keratinocytes in culture. J. Invest. Dermatol. 90: 475-479.
3. Michel, S; Courseaux, A; Miguel, C; Bernardon, J.M; Schmidt, R; Shroot, B; Thacher, S.M; and U. Reichert (l991) Determination of retinoid activity by an enzyme-linked immunosorbent assay. Anal. Biochem. 192: 232-236.
4. Huber, M; Rettler, I; Bernasconi, K; Frenk, E; Lavrijsen, S; Ponec, M; Bon, A; Lautenschlager, D; Schorderet, D; and Hohl, D. (1995) Mutations of Keratinocyte Transglutaminase in Lamellar Ichthyosis. Science. 267: 525-527.
anti-Human Keratinocyte Transglutaminase, Mouse MAb
Catalog No: BT-621
Quantity: 0.5ml (100 sections)
FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.
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