Endothelial Mitogen (ECGS)

Catalogue Number : BT-203

Alternate Names : EM; ECGS

Application Notes : EM (also known as endothelial cell growth supplement) stimulates and supports the growth of vascular endothelial cells and keratinocytes. Additionally, it is used to improve the growth and supplement and/or reduce serum levels in endothelial cell, Balb/c 3T3, and hybridoma cell cultures. Endothelial Mitogen can be used for Human Mammalian Vascular Endothelial Cells at concentrations of 0.1-0.3mg/mL of serum supplemented media 0.01-0.1mg with heparin. Endothelial Mitogen can be used for Hybridoma Cell Cloning at concentrations of 0.025-0.15mg/ml of serum supplemented media. Endothelial Mitogen can be used for Balb/C-3T3 Cells at concentrations of 0.2mg/mL of serum supplemented media.

Concentration : 25-200 μg/mL

Formulation Details : Powder

Pack Size : 50 mg

pH : 7.4

Physical Appearance : Lyophilized orange/pink powder

Presentation : The product was lyophilized from a solution of 0.1m NaCl and 5 mm NaH₂PO₄; pH 7.4 following filtration. | Lyophilized Powder

Protein or Enzyme Type : Growth Factors

Research Areas : Growth Factors Receptors

Solubility : Soluble in water, Reconstitute with 5-10 mL of sterile physiological saline mix gently and transfer to a sterile, screw-cap culture tube. Dilute to a final concentration of 50-300 μg/mL in the appropriate serum supplemented media. Solutions are stable 2 weeks at 4 °C and 1 month at -20 °C

Source : Bovine hypothalamus

Sterility : Sterile | Filtration 0.1 micron membrane for removal of viable mycoplasma.

Storage and Handling : Store at -20°C.

Shelf Life : 2 Years (At -20C Lyophilized)