Salt Active Nuclease

This enzyme will allow for removal of nucleic acids in a traditional protein buffer regime – ie the protein is well protected while the nucleic acids are fully removed. Digests DNA and RNA in approximately a 10:1 ratio. Applications in removal of contaminating nucleic acids from purified enzyme/protein preps as well as inline digestion of nucleic acids in protein purifications. The digestion can take place at high salt conditions.

Main advantages with Salt Active Nuclease :

• Non specific endonuclease
• Optimum activity at high salt concentration (0.5 M NaCl)
• Active at low temperatures (20% at 6ºC)
• Broad pH range
• Temperature stable

Applications :
» Complete removal of DNA
» Viscosity reduction

Concentration is 25units per ul

200ul = 5000units

1000ul = 25000units